Medical science is advancing in leaps and bounds. New treatments, drugs and procedures are introduced regularly. One of the main areas of studies remains the development of new tests that will help physicians to diagnose diseases quicker. The earlier a condition is diagnosed the bigger the chance that it can be treated successfully. ELISA kits have done much to make the diagnosis of several conditions both easier and quicker.
Also known as enzyme immunoassay, or EIA for short, this laboratory technique is used to measure the concentration of antibodies or antigens in a solution, normally a blood sample. The ability to determine these concentrations is extremely important in the diagnostic process. Among the main advantages of this technique, when compared with others, is that it is very easy to carry out and a large number of tests can be processed simultaneously.
The importance of being able to detect the presence of antibodies or antigens cannot be overemphasized. Antibodies are proteins that the body produces when a harmful substance in the body is detected. These harmful substances are called antigens. By being able to identify the type of antibody produced by the body, physicians can accurately determine the type of harmful antigens that triggered the production of the antibodies.
Patients need not fear this simple test. It only entails the drawing of a small blood sample. This sample is then added to specific antigens in a petri dish. If there is a reaction between the blood sample and the antigen, then the presence of antibodies is confirmed. In fact, the antibodies will combine with the antigens. By adding enzymes it is possible to determine the amount of the antibodies in the sample.
A lot of information can be gained from an EIA test. This is true even when the simplest form of the test is used. Yet it is possible to administer more complex tests, such as the direct EIA test. In this case a multiwall coats the antigen. This makes it difficult for the antibody to recognize the antigen. Recognition only takes place when very specific enzymes are introduced.
The indirect IEA test is another example of how this protocol can be used in an advanced manner. In this instance the antigen is actually covered by a polystyrene plate. This forces the antibodies to go through two distinct stages before the antigen is detected. This test allows technicians to identify both primary and secondary antibodies. This is valuable because it may eliminate the need for further expensive tests.
The EIA sandwich test is considered to be suitable for complex samples, especially because the sample does not need to be purified prior to measurement. In this test antibodies are made up in pairs and compared with each other. The test is often done when both direct and indirect detection methods are required. It is an extremely accurate test and is done when serious diseases are suspected.
When all is said and done it is still the early diagnosis of a disease that provides the best chance of recovery and successful treatment. That is why tests such as EIA are so important. They allow doctors to identify the potential or existence of disease at an early stage. This gives the patient a better chance.
Also known as enzyme immunoassay, or EIA for short, this laboratory technique is used to measure the concentration of antibodies or antigens in a solution, normally a blood sample. The ability to determine these concentrations is extremely important in the diagnostic process. Among the main advantages of this technique, when compared with others, is that it is very easy to carry out and a large number of tests can be processed simultaneously.
The importance of being able to detect the presence of antibodies or antigens cannot be overemphasized. Antibodies are proteins that the body produces when a harmful substance in the body is detected. These harmful substances are called antigens. By being able to identify the type of antibody produced by the body, physicians can accurately determine the type of harmful antigens that triggered the production of the antibodies.
Patients need not fear this simple test. It only entails the drawing of a small blood sample. This sample is then added to specific antigens in a petri dish. If there is a reaction between the blood sample and the antigen, then the presence of antibodies is confirmed. In fact, the antibodies will combine with the antigens. By adding enzymes it is possible to determine the amount of the antibodies in the sample.
A lot of information can be gained from an EIA test. This is true even when the simplest form of the test is used. Yet it is possible to administer more complex tests, such as the direct EIA test. In this case a multiwall coats the antigen. This makes it difficult for the antibody to recognize the antigen. Recognition only takes place when very specific enzymes are introduced.
The indirect IEA test is another example of how this protocol can be used in an advanced manner. In this instance the antigen is actually covered by a polystyrene plate. This forces the antibodies to go through two distinct stages before the antigen is detected. This test allows technicians to identify both primary and secondary antibodies. This is valuable because it may eliminate the need for further expensive tests.
The EIA sandwich test is considered to be suitable for complex samples, especially because the sample does not need to be purified prior to measurement. In this test antibodies are made up in pairs and compared with each other. The test is often done when both direct and indirect detection methods are required. It is an extremely accurate test and is done when serious diseases are suspected.
When all is said and done it is still the early diagnosis of a disease that provides the best chance of recovery and successful treatment. That is why tests such as EIA are so important. They allow doctors to identify the potential or existence of disease at an early stage. This gives the patient a better chance.
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